Phage Display Screening
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Waffo, Alain Bopda
The Chemical Genomics Core Facility (CGCF) is adding phage display capability to assist research projects requiring peptide domain screening. They will employ both the traditional DNA M13 phage as well as the RNA Qβ phage for screening. An advantage of the Qβ phage is the ease with which genetic modifications can be made. Qβ phages will be used to display library of peptides (8 to 50-mer) and to screen over 1 million targets. They will be able to assist scientists with the design, experimentation, preliminary result analyses, sequencing data, amplification, reconstitution, and storage of the obtained hybrid phages of interest. In this seminar, Dr. Alain Bopda Waffo discusses the practical methodology and application of phage display as well as newly developed biopanning techniques.
Indiana University School of Medicine; Indiana Clinical and Translational Sciences Institute
Ruth Lilly Medical Library
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